Vol 4 No 1 (2018): Current Issue
Research Article

Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Irradiated Bone Marrow-Derived Mesenchymal Stem Cells

Kawata Y
Department of Oral and Maxillofacial Surgery, Saitama Medical University, Saitama, Japan
Ikami E
Department of Oral and Maxillofacial Surgery, Saitama Medical University, Saitama, Japan
Nojima J
Department of Oral and Maxillofacial Surgery, Saitama Medical University, Saitama, Japan
Kokabu S
Division of Molecular Signaling and Biochemistry, Department of Health Promotion and Kyushu Dental University, Kitakyushu, Fukuoka, Japan
Yoda T
Department of Maxillofacial Surgery, Tokyo Medical and Dental University, Tokyo, Japan
Sato T
Department of Oral and Maxillofacial Surgery, Saitama Medical University, Saitama, Japan
Published October 8, 2018

Abstract

Adipose-derived Mesenchymal stem cells have emerged as an attractive alternative source of cell therapy. While radiation therapy is an important application for head and neck cancer, the effect of adipose-derived mesenchymal stem cells on irradiated bone marrow-derived Mesenchymal stem cells is still unclear. Herein, we explored how clinical total radiation dose affect gene expression related with differentiation on murine bone marrow-derived mesenchymal stem cells and how murine adipose-derived mesenchymal stem cells affect irradiated murine bone marrow-derived mesenchymal stem cells. The clinical total radiation dose upregulates osterix mRNA expression. Moreover, adiposederived mesenchymal stem cells dramatically promoted the upregulation of osterix mRNA expression whereas inhibited NFATc1 mRNA expression. Taken as a whole, irradiated bone marrow-derived mesenchymal stem cells co-cultured with adipose-derived mesenchymal stem cells may exhibit osteogenic property.