The Neuroprotective properties of Gastrodiae Rhizoma Water Extract and its Underlying Mechanisms in Experimental Ischemic Stroke
- Ischemic stroke,
Copyright (c) 2019 Hung et al.
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Aims: Gastrodiae Rhizoma has a long history in treating a neurological disorder such as headache, epilepsy, dizziness, and hemiplegia. The present study aims to determine the efficacy of Tianma extract on neuroprotection with regards to ischemic stroke.
Methods: Anti-oxidative and anti-apoptotic effects of Tianma extract were investigated by using oxygen-glucose deprivation/reperfusion (OGD) on PC12 cells. The anti-inflammatory effect of Tianma extract was revealed by using lipopolysaccharide (LPS)-induced production of various inflammatory mediators and cytokines on RAW 264.7 cells. Middle cerebral artery occlusion on the SD rat was adopted to mimic ischemic stroke for evaluating the efficacy of Tianma water extract on neuroprotection.
Results: The results demonstrated that Tianma water extract could rescue PC12cell survival by inhibiting ROS production. Meanwhile, Tianma also possessed the anti-apoptotic effect by regulating Bcl-2 and Bax apoptotic proteins and reducing the cleavage of PARP. Tianma extract also exerted inhibitory effects on LPS-induced NO production via suppression on iNOS and COX-2 expression, and also inhibited LPS-induced production of IL-6 and TNF-α in RAW 264.7 cells. Further rat MCAO experiment showed the anti-oxidative and anti-inflammatory effects of Tianma water extract by increasing anti-oxidative enzyme activities and ameliorating inflammatory cytokines, respectively.
Conclusions: It is suspected that these neuroprotective properties of Tianma could help patients with ischemic stroke.