Integrative Biomedical Sciences

Vol. 1 No. 2 (2015)
Research Article

Association of Serum Antibody Levels against TUBB2C with Diabetes and Cerebral Infarction

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  • Takaki Hiwasa,
  • Xiao-Meng Zhang,
  • Risa Kimura,
  • Toshio Machida,
  • Kenichiro Kitamura,
  • Rika Yamazoe,
  • Mitoshi Kunimatsu,
  • Seiichiro Mine,
  • Eiichi Kobayashi,
  • Yasuo Iwadate,
  • Naokatsu Saeki,
  • Minoru Takemoto,
  • Kazuki Kobayashi,
  • Harukiyo Kawamura,
  • Ryoichi Ishibashi,
  • Ken-ichi Sakurai,
  • Masaki Fujimoto,
  • Koutaro Yokote,
  • Yo Iwata,
  • Takashi Nakayama,
  • Jun-ya Harada,
  • Yoshio Kobayashi,
  • Mikiko Ohno,
  • Po-Min Chen,
  • Eiichiro Nishi,
  • Mitsuhiro Yokota,
  • Ikuo Kamitsukasa,
  • Takeshi Wada,
  • Akiyo Aotsuka,
  • Masahiro Mori,
  • Akiyuki Uzawa,
  • Mayumi Muto,
  • Kazuo Sugimoto,
  • Satoshi Kuwabara,
  • Ken-ichiro Goto,
  • Ryutaro Matsumura,
  • Hirotaka Takizawa,
  • Hideaki Shimada,
  • Masaaki Ito,
  • Hao Wang,
  • Akiko Taira,
  • Emiko Arita,
  • Katsuro Iwase,
  • Takashi Kudo,
  • Hirofumi Doi,
  • Rika Nakamura,
  • Go Tomiyoshi,
  • Natsuko Shinmen,
  • Hideyuki Kuroda
Takaki Hiwasa
Department of Biochemistry and Genetics, Chiba University, Graduate School of Medicine, Chiba

Published 2015-05-18

Copyright Notice

Abstract

Cerebral infarction (CI), cardiovascular disease (CVD), diabetes mellitus (DM) and chronic kidney disease (CKD) are atherosclerosis-related diseases, which are major causes of health damage. For early and sensitive diagnosis, development of novel biomarkers is expected and of significant practical importance. First screening was carried out by phage expression cloning to identify antigen proteins recognized by serum IgG antibodies in patients with atherosclerosis. RPA2, LRPAP1, EEF1A1, SPOCK1, LOC729260, tubulin beta 2C (TUBB2C) and KIAA0020 markers were identified. We then compared the serum antibody levels against the candidate proteins between healthy donors (HD) and patients with CI, CVD, DM, or CKD by Alpha (amplified luminescent proximity homogeneous assay)-LISA method. The results showed that the serum TUBB2C antibody levels were significantly higher in patients with CI, DM, or CKD than those in HD. Using the average + 2SD of HD as the cut-off value, the positive thresholds of TUBB2C antibody markers were 14.8% in CI, 25.8% in DM, and 18.3% in CKD. TUBB2C antibody levels were well correlated with artery stenosis degrees such as plaque score, maximum intima-media thickness and cardio ankle vascular index. Consequently, TUBB2C antibody markers are useful to diagnose atherosclerosis, DM, and CKD, and can be applied to the prediction of the onset of CI. The serum anti-TUBB2C antibody markers are useful for the diagnosis of DM and CKD.

 Keywords: Atherosclerosis; Diabetes mellitus; Chronic kidney disease; Cerebral infarction; Cardiovascular disease; Antibody biomarker

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