2nd World Congress and Expo on Nanotechnology and Material Science April 04-06, 2016 at Dubai, UAE
Conference Proceedings

Poster Cell-Instructive Hydrogel for a Control of Stem Cell Phenotype

Kuen Yong Lee
Department of Bioengineering, Hanyang University
Hyo-Seok An
Department of Bioengineering, Hanyang University
Jae-Won Lee
Department of Bioengineering, Hanyang University

Published 2018-01-01


Controlling the cell phenotype using synthetic extracellular matrices such as hydrogels has been widely investigated, andespecially a use of cell-instructive systems is essential to regulate the phenotype of mesenchymal stem cells (MSCs). Inthis presentation, we report a biomimetic system that provides a stem cell niche using cell-instructive alginate-based microgels.Alginate microgels were prepared by the water-in-oil emulsion method, solidified in the presence of calcium chloride, and thenmodified with a peptide derived from E-cadherin receptor. Chondrogenic differentiation of MSCs is typically influenced by cellaggregate formation and cadherin is a key factor in mediating cell-cell interactions. These spherical microgels with a similar sizeto that of cells formed an aggregate in the presence of MSCs, resulting in excellent cell viability and enhanced chondrogenesisin vitro. This approach to controlling cell-interactive features of hydrogels may be useful in 3-D stem cell culture and tissueengineering applications.