The purpose of the present investigation was the formulation and characterization of mucoadhesive sustained release microsphere of antidibetic drug Acarbose that would adhere in mucosa and release continuously to provide long term effect. There was various formulations of Acarbose were prepared by solvent evaporation technique using hydroxypropyl methylcellulose (HPMC), Sodium Alginate, Carbopol, Xanthan gum, Guar gum as a polymer. The prepared mucoadhesive microspheres were evaluated for particle size, surface morphology, drug entrapment efficiency, Drug content, buoyancy percentage and In-vitro drug release, In-vitro adhesion test and stability studies. The particle was found to be discrete and spherical with the average particle size in the range of 105.5 to 413.5μm. As the concentration of polymers increases it affects the various evaluation parameters like particle size, in-vitro drug release and In-vitro adhesion. The floating microspheres of optimized formulation exhibited the prolonged release of 85.8% in continuous manner up to 12 hrs. It is concluded that the optimized formulation of Acarbose mucoadhesive microspheres can be selected for sustained drug delivery system for improved bioavailability.