2019: Articles in press
Research Article

Brown Adipose Tissue Remodeling Precedes Cardiometabolic Abnormalities Independent of Overweight in Fructose-Fed Mice

Machado TQ
Laboratório Multiusuario de Pesquisa Biomédica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova Friburgo, Nova Fribrugo, RJ, Brazil
Pereira-Silva DC
Laboratório Multiusuario de Pesquisa Biomédica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova Friburgo, Nova Fribrugo, RJ, Brazil
Goncalves LF
Laboratório Multiusuario de Pesquisa Biomédica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova Friburgo, Nova Fribrugo, RJ, Brazil
Fernandes-Santos C
Laboratório Multiusuario de Pesquisa Biomédica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova Friburgo, Nova Fribrugo, RJ, Brazil
Published July 23, 2019

Abstract

Background: Body fat distribution is a risk factor for several health conditions, although the literature shows that excess body fat is not always associated with cardiometabolic abnormalities in all subjects.
Objectives: To investigate glucose, lipid, and hepatic metabolism, along with white (WAT) and brown adipose tissue (BAT) remodeling in a mice model of short-term fructose feeding.
Methods: Male C57BL/6 mice (n = 10-11/group) were fed for four weeks with control diet (AIN93-M) or experimental diets rich in glucose or fructose. We investigated body weight, body adiposity, blood glucose, lipid and hepatic parameters, and WAT and BAT histopathology.
Results: Fructose feeding did not promote either weight gain or adipocyte hypertrophy of visceral and subcutaneous WAT depots, but the fat was redistributed toward visceral depots. Fructose-fed mice did not show glucose, lipid, and hepatic metabolic dysfunction, except for an elevation in total cholesterol and hepatic weight. BAT mass did not increase, and it was proportionally reduced compared with visceral WAT in fructose feed mice. BAT suffered early adverse morphological remodeling, characterized by increased lipid deposition and enlargement of intracellular lipid droplets.
Conclusion: Short-term fructose feeding redistributes fat among WAT depots, alters the ratio between BAT and visceral WAT, and promotes BAT adverse remodeling, characterized by enlarged intracellular lipid droplets.